Figure 4: Assays for LCN2-mediated inhibition of E. coli growth in the presence or absence of c-di-GMP. | Nature Communications

Figure 4: Assays for LCN2-mediated inhibition of E. coli growth in the presence or absence of c-di-GMP.

From: Cyclic diguanylate monophosphate directly binds to human siderocalin and inhibits its antibacterial activity

Figure 4

(a) ITC assays for the interaction between Fe-Ent and rLCN2 protein. Original titration data and integrated heat measurements are shown in the upper and lower plots, respectively. (b) Assays for changes in E. coli growth mediated by the human rLCN2 protein in the presence or absence of c-di-GMP. c-di-GMP could rescue the growth inhibition by rLCN2, but hardly by rLCN2-W79A. Addition of FeCl3 or Fe-Ent could rescue rLCN2-mediated growth inhibition. The control nucleotide, c-di-AMP or cGAMP, could not rescue rLCN2-mediated growth inhibition. Bacterial counts were determined at two representative time points, 18 h (top panel) and 24 h (bottom panel). Double asterisks (**) represent significant difference between two groups (P≤0.01, two-tailed Student’s t-test).

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