Figure 5: Crystal structure of P[11] bovine RV (BRV) VP8*.

(a) Sequence alignment of P[11] VP8*s of various representative P[11] human RV (HRV) and BRV strains. The consensus amino acids are coloured using Clustal protein colour scheme in Jalview⁵⁰. The black * indicates the residues recognizing both LNnT and LNT, and the red * indicates those only bind LNT in P[11] HRV N155. The key amino acid changes between HRV and BRV strains are marked by black boxes. (b) Structural overlay of the structure of P[11] HRV N155 VP8* coloured in grey with that of the P[11] BRV B223 VP8* in pink. The significant structural variations of the J-K loops are indicated by a black box. (c) The J-K loop of P[11] HRV VP8* shows the hydrogen bond interactions between the amino acids leading the loop projecting the strand H. (d) The J-K loop of P[11] BRV VP8* presented in the same orientation and scale as in c. All the residues present in the J-K loop are shown as stick model.