Figure 6: Interactions between LNnT and P[11] BRV VP8*.

(a) Structure of P[11] BRV VP8*(pink) in complex with LNnT (type II). The VP8* protein is shown in grey ribbon and surface (inlet figure) representations. The glycans are shown in stick representation with the β-D-Galactose (Gal) coloured yellow, the N-acetyl-D-glucosamine (GlcNAc) in green and the β-D-Glucose (Glc) in blue. Molecular interactions of LNnT with BRV N155 P[11] VP8* were analysed using LIGPLOT (also see Supplementary Fig. 2c). (b) Superimposition of the structure of P[11] BRV VP8*(pink) or P[11] HRV VP8*(grey) in complex with LNnT (type II). The interacting residues are shown in stick model. The interacting residues is presented in stick model, and the glycan residues are labelled as in a. (c) Structural overlay of P[11] BRV VP8*(pink)/LNnT complex with P[11] HRV VP8*(grey)/LNT complex. (d) The schematic diagram of glycan recognition in both HRV and BRV VP8*s shows how the amino acid changes in P[11] BRV lead to loss of binding to type I glycan LNT, and how both VP8*s recognize type II glycan LNnT in a similar manner.