Figure 2: Tamoxifen-induced extracellular trap production is PKCζ dependent. | Nature Communications

Figure 2: Tamoxifen-induced extracellular trap production is PKCζ dependent.

From: Tamoxifen augments the innate immune function of neutrophils through modulation of intracellular ceramide

Figure 2

(a) To determine whether PKC is required for TAM-induced NET production, neutrophils were pre-incubated with (a) the general PKC antagonist chelerythrine chloride (n=8) or (b) the more selective inhibitors Gö 6976 and Gö 6983 (n=9) before treatment with 10 μM TAM. (c) Neutrophils were pre-incubated with PKCζ pseudosubstrate (PKCζ-PS) to specifically evaluate the role of PKCζ in TAM-induced NET production. (d) Immunostaining/confocal microscopy was used to visualize translocation of PKCζ in human neutrophils in response to TAM (10 μM) treatment (blue: 4′,6-diamidino-2-phenylindole; green: PKCζ); images representative of three independent experiments are shown (scale bar, 10 μm). (e) Bone marrow-derived neutrophils were collected from PKCζ knockout mice to determine TAM-induced NET production in comparison with wild-type (WT) littermate controls (n=4–5). Where applicable, results were analysed by one-way analysis of variance and post hoc Newman Keuls test. *P<0.05 versus control values.

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