Figure 4: IP-HPLC analyses of products.
From: Formation of oligopeptides in high yield under simple programmable conditions
(a) IP-HPLC chromatograms of a standard glycine condensation reaction, showing product oligomer length increasing with dehydration time (between 1 and 24 h). (b) Similar analysis of the solid precipitate formed during the reaction after two dehydration–hydration cycles, taken up in a 0.1% trifluoroacetic acid (TFA)/H2O solution, showing longer (sparingly soluble) oligomers. In both a and b, Glyn oligomer peaks are labelled with the corresponding value of n; assignment of the peaks was established by comparison with Gly standards and by separate MS confirmation.
