Figure 1: Morphological evolution of cell growth domains in fission yeast.
From: Wall mechanics and exocytosis define the shape of growth domains in fission yeast
(a) Transmitted light images illustrating the changes in OE and NE shape in a fission yeast cell throughout interphase. Left, cell immediately before NETO. Images are maximum intensity projections of z-stacks comprising the most equatorial 2 μm of the cell. Scale bar, 5 μm. (b) Average meridional curvature of the NE (red line) and OE (green line). The coloured areas around the averages correspond to the standard deviations. n=35 OEs and n=35 pre-NETO NEs were averaged. (c) Cell contours extracted from the time-lapse sequence in a. (d) Curvature kymographs showing a pointy OE keeping its curvature (top) and a hemispherical NE evolving into a pointy OE (bottom). The plots, corresponding to the ends of the cell depicted in a, display the meridional curvature as a ‘heat map’ during 3 h with a 2-min resolution. One of n=25 OEs and one of n=18 NEs are shown. (e) Schematic illustration of the three morphogenetic transitions observed in S. pombe: (i) the deformation of the flat post-cytokinesis septum into a hemispherical NE (first arrow), (ii) the growth of the hemispherical NE into the pointy OE (second arrow) and (iii) the steady growth of the OE (third arrow).
