Figure 3: Morphogenetic potential of cell end-distributed factors.
From: Wall mechanics and exocytosis define the shape of growth domains in fission yeast
(a) Diagrammatic representation of the function of 11 key factors involved at different levels of the polarized growth cascade (black, polarity factors; green, exocytosis; blue, glucan synthesis). (b) Cortical OE distribution in vivo of the factors fluorescently labelled. The images are OAI sum projections (12 frames every 10 s). (c) Top panels: The symmetrized average OE distribution of each GFP-labelled marker (n=29). The darker grey area corresponds to the full-width at half-area (FWHA), a parameter quantifying the spread of a given marker in the apical membrane; the lighter grey area corresponds to the full-width at 95% area (FW95A), which defines the shape of the distribution along the membrane (see Supplementary Fig. 3 for the distributions’ standard deviations). Bottom panels: Modelled OE morphologies obtained when using the average factor distribution as a proxy for new wall incorporation. Note how different factors belonging to similar machineries have similar distributions and lead to similar geometries, as quantitatively illustrated by their respective branch length in the dendrogram. The Areal distribution is inferred from the strain rate profiles of Fig. 2.
