Figure 4: Wall mechanics and exocytosis suffice to drive growth domain morphology.
From: Wall mechanics and exocytosis define the shape of growth domains in fission yeast
(a) Top: Sum intensity projection images of pal1 Δ and wild-type cells stained with calcofluor white, which binds to the linear chains of β-1,3 glucan, or expressing RFP-Bgs4 and Sec6-GFP. Scale bars, 5 μm. Bottom: Quantitation of the fluorescence intensity of each of the three reporters in pal1 Δ and wild-type. (b) Sum intensity projection images showing rga2Δ (top), wild-type (middle) and rga4Δ (bottom) cells expressing CRIB-3GFP, Sec6-GFP or RFP-Bgs4. Scale bars, 5 μm. Transmitted light images of the mutants and wild-type are depicted on the left. (c) Plots illustrating the average full-width half-area (FWHA) of the distribution of Cdc42 markers (CRIB-3GFP and Scd2-GFP; left), exocytosis markers (Sec6-GFP, Exo70-GFP, For3-3GFP and GFP-Syb1) and the glucan synthesis marker RFP-Bgs4 against the cell width in the mutants rga2Δ (left within each plot) and rga4Δ (right within each plot) and the wild-type (middle within each plot). n=26 cells per condition. The standard deviations are represented as crosses emerging from each average value (coloured shapes). The dashed line corresponds to the observed relationship between the FWHA of the wild-type wall expansion strains and the cell radius.
