Figure 5: Exocytosis pattern and growth domain morphogenesis are causally linked.
From: Wall mechanics and exocytosis define the shape of growth domains in fission yeast
(a) Cartoon describing the strategy chosen to re-direct GFP-Syb1-containing vesicles to the whole extension of the GBP-mCherry-CaaX-containing plasma membrane. (b) Forced re-directioning of exocytic vesicle fusion to broader areas of the plasma membrane causes proportional morphological changes to growth domains. Left: Images showing misshapen cells because of the ectopic distribution of GFP-Syb1 in a GBP-mCherry-CaaX background. Right: Plots depicting the average (thick line) curvature and GFP-Syb1 apical distribution of n=30 OEs (the thin lines represent the standard deviation). (c) Images showing the distribution of GBP-mCherry-CaaX in the absence of GFP-Syb1 (left) and the canonical curvature of wild-type OEs (right; this corresponds to the data displayed in Fig. 1b; n=29 OEs). (d) Images showing the distribution of GFP-Syb1 in the absence of GBP-mCherry-CaaX (left) and the canonical OE distribution of GFP-Syb1 in wild-type cells (right; this corresponds to the data displayed in Supplementary Fig. 3; n=29 OEs). Scale bars, 5 μm.
