Figure 5: In vivo characterization of the redox-switch regulatory mechanism of the CaTHL.
From: Redox-switch regulatory mechanism of thiolase from Clostridium acetobutylicum
The thlca gene was overexpressed in the biphasic C. acetobutylicum type strain (ATCC 824), to see if the overexpression of CaTHL affects the metabolism with respect to metabolic flux distribution and the acidogenic to solventogenic phase transition. Anaerobic batch fermentation profiles of the biphasic C. acetobutylicum ATCC 824 (a) and the ATCC 824 strain overexpressing the CaTHL (b) are shown. Symbols are: acetic acid (open squares), butyric acid (filled diamonds), lactic acid (open circles), pH (crosses), OD600 (filled squares), glucose (filled circles), acetone (open diamonds), ethanol (open triangles) and n-butanol (closed triangles). Fermentations were performed at least in duplicates showing reproducibility, and the profiles shown are that of one representative fermentation.
