Figure 1: The GNP. | Nature Communications

Figure 1: The GNP.

From: An automated Genomes-to-Natural Products platform (GNP) for the discovery of modular natural products

Figure 1

(a) The automated GNP pipeline processes submitted sequences to identify NRPS and PKS gene clusters and yield predicted structures. These predictions can be combinatorialized and elaborated to account for biosynthetic promiscuity, creating libraries of hypothetical structures that are used to search LC–MS/MS data and automatically reveal the true genetically encoded natural product. (b) A novel nonribosomal peptide biosynthetic gene cluster identified within S. calvus, alongside the combinatorialized GNP-generated structure prediction. (c) LC–MS/MS chromatogram of an S. calvus culture extract with GNP result indicating a localized genetically predicted structure. (d) Chemical structures of the top-scoring GNP-generated structure prediction (left) and the corresponding natural product, WS9326C (right), depicted with corresponding retention times (RTs) and mass to charge ratios (m/z).

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