Figure 6: Continuous evolution of PT3-active RNAP variants.
From: Development of potent in vivo mutagenesis plasmids with broad mutational spectra
S1030 cells carrying an accessory plasmid (AP) encoding PT3 upstream of M13 bacteriophage geneIII and either DP1 or DP6 were infected with selection phage (SP) carrying wild-type T7 RNAP under conditions in which selection stringency was high (0 ng ml−1 ATc) or moderate (30 ng ml−1 ATc). (a) Total phage titres at 10 and 20 h after lagoon inoculation with the SP encoding T7 RNAP. (b) Titres of phage encoding evolved RNAP variants active on PT3. The limit of detection is 50 p.f.u. ml−1. p.f.u., plaque-forming units.
