Figure 2: Loss of KLF14 induces genome instability.
From: Loss of KLF14 triggers centrosome amplification and tumorigenesis
(a,b) KLF14 KO induces aneuploidy. (a) Cells with >4N DNA content increased in KLF14-KO MEFs. Representative flow cytometry profiles of KLF14-WT and -KO passage 3 (P3) MEFs stained with propidium iodide. Bar graph shows the mean percentage of cells with DNA content >4N. Data represent the mean±s.d. from three separate experiments, **P<0.01. (b) Representative images show Giemsa staining of metaphase chromosome spreads in KLF14-WT and -KO P3 MEFs (scale bar, 10 μm). Graph shows the fraction of MEFs with different numbers of chromosome per cell. One hundred metaphase spreads were counted for each group. Data represent the mean±s.d. from three separate experiments, *P<0.05, **P<0.01. (c) KLF14 KO induces chromosome instability. Spindles and DNA in KLF14-WT and -KO P3 MEFs were stained with α-tubulin (red) and 4,6-diamidino-2-phenylindole (DAPI, blue), and visualized by fluorescence microscopy. Representative images show mitotic chromosomal abnormalities including micronucleus, bridges, laggards and scattering in KLF14-KO MEFs (scale bar, 10 μm). Bar graph shows the percentages of MEFs with mitotic chromosomal abnormalities. More than 100 mitotic cells were counted for each group; data represent mean±s.d., **P<0.01.
