Figure 2: O-GlcNAcylation regulates G6PD enzyme activity and oligomerization.
From: O-GlcNAcylation of G6PD promotes the pentose phosphate pathway and tumor growth
(a) Enzymatic activities of WT and S84V G6PD purified from untreated (Cont), OGT-overexpressing, and thiamet-G (TMG) treated 293T cells (n=4 experiments). Error bars denote the standard deviation of the mean (mean±s.d.). Statistical analysis was performed by one-way analysis of variance and Bonferroni comparison post-test (*P<0.05). (b) Oligomerization state of Flag-tagged WT and S84V G6PD in cells. Crosslinking with glutaraldehyde and followed by immunoblotting detected dimeric and monomeric G6PD. Flag-tagged G6PD protein input was shown in the lower panel. (c) Immunoblots of G6PD glycosylation under different cellular treatments. NS, not significant.
