Figure 3: O-GlcNAcylation of G6PD regulates metabolic flux through the PPP.
From: O-GlcNAcylation of G6PD promotes the pentose phosphate pathway and tumor growth
(a) PPP activity in WT and S84V G6PD replacement cells in the absence or presence of OGT overexpression, as determined by the amount of released 14CO2 from [1-14C]-glucose (n=3 assays). (b) Percentage of glucose flux through the PPP in WT and S84V G6PD replacement cells in the absence or presence of OGT overexpression, as measured by the relative accumulation of singly 13C-labelled lactate from cells metabolically labelled with 1,2-13C-glucose using reverse-phase triple-quadrupole LC–MS (n=3 assays). (c–e) Targeted analysis of abundance of different metabolites in major glucose metabolic pathways: PPP (c), glycolysis (d), and TCA cycle (e) in WT and S84V G6PD replacement A549 cells on OGT overexpression (n=3 experiments). Error bars denote mean±s.d. Statistical analysis was performed by Student’s t-test (*P<0.05).
