Figure 6: BAP1 knockdown partially suppresses breast cancer lung metastasis through KLF5.

(a) Knockdown of either BAP1 or KLF5 decreased HCC1806 cell migration as measured by wound-healing assays. The wound edges are indicated by white lines. Representative images are shown. Scale bar, 100 μm. (b) The quantitative results of a (n=3). The y axis represents percentage of wound closure. Every experimental group was compared with the Lucsh group. Error bars represent s.d. from three repeats. **P<0.01, t-test. (c) Knockdown of either BAP1 or KLF5 decreased HCC1806 cell invasion as measured by Matrigel Transwell assays. The blue dye indicated the transwell cells. Representative images are shown. Scale bar, 100 μm. (d) The quantitative results of c. **P<0.01, t-test. The OD values of transwell cells were measured using a microplate reader. Every experimental group was compared with the Lucsh group. Error bars represent s.d. from three repeats. **P<0.01, t-test. (e) Knockdown of BAP1 in 4T1 cells was confirmed by WB. Knockdown of KLF5 in 4T1 cells was confirmed by RT–quantitative PCR (n=3, mean±s.d. from three experiments with triplicate). The KLF5 siRNA group was compared with the control siRNA group. *P<0.05, t-test. (f) Overexpression of HA-mKlf5 in 4T1 cells was detected by WB. (g) Bioluminescence images of mouse lung metastasis from the four groups 5 weeks after orthotopic injection. (h) The quantitative results of g (n=5, each group). The KLF5si or BAP1si groups were compared with the Controlsi group. The rescue group (BAPsi+HA-klf5) was compared with the KLF5si or BAP1si groups. **P<0.01, mean±s.d., t-test.