Figure 5: rTg4510 brain-derived human tau was stable and propagated even after removal of brain extract from the chamber. | Nature Communications

Figure 5: rTg4510 brain-derived human tau was stable and propagated even after removal of brain extract from the chamber.

From: Neuronal uptake and propagation of a rare phosphorylated high-molecular-weight tau derived from Alzheimer’s disease brain

Figure 5

(a) rTg4510 brain extract (12-month-old, PBS-3,000g) was diluted in culture medium (500 ng ml−1 human tau in final concentration) and added to the 1st chamber of 3-chamber microfluidic neuron device. After 2 days (before tau propagation occurs) or 5 days (after tau propagation occurred, but not yet progressed to the 3rd chamber) of incubation, brain extract was washed out from the 1st chamber and replaced with fresh culture medium. (b,c) Neurons were immunostained for human tau (green) and total (human and mouse) tau (red) at designated time points. (b) Human tau positive neuron was detected in the 2nd chamber (day 8, arrow) even after Tg brain extract was washed out from the 1st chamber at day 2. (c) Human tau was detected in the 3rd chamber axons (arrow) even after Tg brain extract was washed out from the 1st chamber at day 5. Human tau taken up by the 1st chamber neuron was still detectable at day 14 (9 days after removal of Tg brain extract). Scale bar, 50 μm.

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