Figure 6: DNA methylation status of the miR-622 promoter.
From: EZH2-mediated loss of miR-622 determines CXCR4 activation in hepatocellular carcinoma
(a) Scheme depicting the genomic localization of miR-622. TSS is indicated with an arrow. Transcription start site (TSS) locations were predicted using miRStart (http://mirstart.mbc.nctu.edu.tw/)44. The regions analysed by BSP are indicated. (b) The sequence level detail of the miR-622 promoter regions (−3,616 to −3,403, 213 bp); the CpG dinucleotides within this region are numbered as 1–14. (c) The methylation status of the miR-622 promoter in the normal liver tissue (normal) and SK-Hep1, SNU448, HepG2, Hep3b, PLC/PRF/5 and Huh7 cell lines. The open and filled circles indicate the unmethylated and methylated CpGs, respectively. (d) Quantitative PCR (qPCR) analysis of miR-622 expression in indicated cells treated with 5 μmol 5-aza-dC for 72 h (n=3). Student’s t-test, *P<0.05. Error bars in panels are defined as s.d. (e) The methylation status of the miR-622 promoter in 13 pairs of HCC and peritumour tissues. (f) qPCR analysis of miR-622 expression in 13 pairs of HCC and peritumour tissues (left) and in HCC tissues with or without methylation in miR-622 promoter (right; n=3). Student’s t-test, *P<0.05. Error bars in panels are defined as s.d.
