Figure 2: Ablation of p110β blocked MPN development in a cell-autonomous manner. | Nature Communications

Figure 2: Ablation of p110β blocked MPN development in a cell-autonomous manner.

From: A PI3K p110β–Rac signalling loop mediates Pten-loss-induced perturbation of haematopoiesis and leukaemogenesis

Figure 2

(a) Colony-forming assays were performed with 1 × 104 (BM) or 5 × 104 (spleen) cells obtained from corresponding animals. Cells were cultured in M3434 medium. Colonies were counted 7 days after incubation. Data are represented as mean±s.e.m. (n=3, in duplicates). *P<0.05, **P<0.01, ***P<0.001. (b) BM cells were isolated from CD45.2+ WT, PtenΔ/Δ and PtenΔ/Δ;p110βΔ/Δ animals and transplanted into CD45.1+ mice as shown in the figure (n=10 for each group). (c) The myeloid cell reconstitution potential of the CD45.2+ cells was measured at regular intervals for 16 weeks. WT and PtenΔ/Δ;p110βΔ/Δ-derived myeloid cells have a stable amount of CD45.2+ myeloid cells; however, PtenΔ/Δ-derived myeloid cells expanded significantly and reached to 60% at week 16 (n=10 for all groups, **P<0.001). Two-way ANOVA test was applied to compare the myeloid cell potential.

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