Figure 3: Jag1 induces the downregulation of the endothelial signature.
From: Notch signal strength controls cell fate in the haemogenic endothelium
(a) Microarray validation using qRT–PCR. Expression of a panel of endothelial-related genes on E11.5 CD31+CD45−Ter119− AGM cells incubated 2 h on OP9-Jag1. Quantification was performed in Kit−, Kit+, Kit−J and Kit+J. The bars represent the average expression level of four to six replicates from two independent experiments, normalized to Kit− expression. Average fold change (±s.d.) is represented. Student’s t-test for comparisons of Kit+, Kit−J and Kit+J populations with Kit− population was performed (*P≤0.05; **P≤0.01; P≤0.001). (b) Expression of haematopoietic genes on E10.5 and E11.5 Kit− and Kit−J populations (2 h on OP9-Jag1). The bars represent the average (±s.e.m.) expression level of six to nine replicates from three or four independent experiments, normalized to Kit− expression. Statistical significance was assessed by Student’s t-test (*P≤0.05; ***P≤0.001 when significant). nd, not detected.
