Figure 1: IL-4/IL-13 alter the miRNA expression profiles in macrophages. | Nature Communications

Figure 1: IL-4/IL-13 alter the miRNA expression profiles in macrophages.

From: miR-142-5p and miR-130a-3p are regulated by IL-4 and IL-13 and control profibrogenic macrophage program

Figure 1

(a) Primary human macrophages derived from peripheral blood monocytes were treated for 48 h with 20 ng ml−1 IL-4 or IL-13, and the miRNA expression profiles were analysed by microarray. The mean fluorescence intensity was calculated as the average for three replicates. The results are presented as the miRNA ratio versus the untreated cells (n=3 per group, fold change >2, P<0.05 by two way ANOVA). (b) Venn diagram of all miRNAs with different expression in the cells treated as in (a). Red indicates the overlap between IL-4-regulating miRNAs and IL-13-regulating mRNAs. (c) Absolute quantification of the expression of the indicated miRNAs at 48 h following IL-4/IL-13 treatment as determined by qRT–PCR (mean±s.e.m., n=4; *P<0.05; **P<0.01; ***P<0.001, compared with untreated macrophages by two-tailed Student’s t-test). ANOVA, analysis of variance.

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