Figure 2: miR-142-5p and miR-130a-3p regulate M2 activation of macrophages. | Nature Communications

Figure 2: miR-142-5p and miR-130a-3p regulate M2 activation of macrophages.

From: miR-142-5p and miR-130a-3p are regulated by IL-4 and IL-13 and control profibrogenic macrophage program

Figure 2

(a–d) Human macrophages were transduced with nc, miR-142-5p ASO or miR-130a-3p mimics or both using lentiviral vectors. After 24 h, the cells were treated with IL-4 for 48 h. (a) The schematics of the approach. (b) Expression of CD206 and CD36 in macrophages as determined by flow-cytometry analysis. The representative histograms and quantitation of the MFI are shown (mean±s.e.m., n=4 independent experiments; *P<0.05; **P<0.01; ***P<0.001. P values were obtained using two-tailed Student’s t-test). (c) Representative images of fluorescent fibronectin(FN)/DAPI staining in macrophages. Scale bar, 20 μm. Fluorescence intensity was quantitated by ImageJ software and normalized to control levels (mean±s.e.m., n=3 independent experiments; *P<0.05; **P<0.01. P values were obtained using two-tailed Student’s t-test). (d) Cytokine levels in the media of macrophages (mean±s.e.m., n=4 independent experiments; *P<0.05; **P<0.01; ***P<0.001. P values were obtained using a two-tailed Student’s t-test). MFI, mean fluorescence intensity; nc, negative control.

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