Table 1 Origin density in Mcm3+/++/+ and Mcm3-deficient EBs.

From: Replication stress caused by low MCM expression limits fetal erythropoiesis and hematopoietic stem cell functionality

 

R1

R2

R3

R4

 

+/++/+

L/L

+/++/+

L/L

+/++/+

L/L

+/++/+

L/L

n. oris

327

382

325

344

285

292

285

250

DNA, Mb

141,3

118,4

110,9

108,7

89,91

84,24

85,29

69,10

ori, Mb

2,32

3,23

2,93

3,16

3,17

3,47

3,34

3,62

  1. Proliferating EBs derived from the fetal liver of Mcm3+/+ or Mcm3Lox/Lox (L/L) embryos were labeled with CldU and IdU and isolated at different maturation stages (R1-R4) by flow cytometry after CD71/Ter119 staining. Origin density was calculated by measuring the number of origin structures in multiple microscopy fields, until a minimum of 250 origins were scored per condition, and the total length of DNA in every fiber containing at least one replication structure.
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