Figure 2: Gas-phase stabilization of soluble and membrane proteins by ligand molecules.
From: Quantifying the stabilizing effects of protein–ligand interactions in the gas phase
For each system, a typical mass spectrum is shown, with the derived stabilization of the protein by the ligand(s) and a representative crystal structure of the protein. (a) Streptavidin with B4F (stabilization for 16+ charge state, PDB entry 1STP with bound biotin shown as spheres); (b) transthyretin with L-thyroxine (T4) (stabilization for 15+ charge state, PDB entry 2ROX with bound T4 shown as spheres); (c) S. aureus MscL with the lipids L-α-PG, CDL and lysyl-PG (stabilization for 12+ charge state) (structure shown of M. tuberculosis MscL, PDB entry 2OAR). (d) The multidrug and toxic compound extrusion protein from P. furiosus with CDL and PG (stabilization for 11+ charge state, PDB entry 3VVN). Reported are average and s.e.m. from repeated measurements (n=3).
