Figure 6: Solvent-exposed mIgG-tail induces the excessive recruitment of prominent BCR signalling microclusters into the immunological synapse.

(a) TIRFM images to show the synaptic accumulation of BCRs in Ramos B cells expressing mIgG-tail or mIgG-Linker25-tail that were placed on surrogate antigens presenting PLBs. (b,c) Statistic quantification for the TFI of accumulated BCRs in the immunological synapse (b) and for the size of the contact area (c) of Ramos B cells expressing mIgG-tail and mIgG-Linker25-tail as represented in a. (d–f) Representative TIRFM images were given to show the synaptic recruitment of pSyk (d) pBLNK (e) pPI3K (f) in Ramos B cells expressing mIgG-tail or mIgG-Linker25-tail. (g–i). Statistic quantification were given to compare the synaptic accumulation of signaling molecules of pSyk (g) pBLNK (h) and pPI3K (i) in Ramos B cells expressing mIgG-tail or mIgG-Linker25-tail. In all the figures, given is one representative data out of three independent experiments. Bars indicate mean+s.e.m. Scale bar, 1.5 μm (a,d–f). Two-tailed t-tests were used for the statistical comparisons. ***P<0.001; **P<0.01.