Figure 7: Solvent-exposed mIgG-tail induces the inflated Ca²⁺ mobilization and hyperproliferation in mouse primary B cells.

(a) Analysis of the Ca²⁺ mobilization in mouse primary splenic B cells retrovirally expressing mIgG-tail, mIgG-tailless or mIgG-Linker25-tail. One representative data out of three independent experiments is shown. (b,c) A representative proliferation profile was given in mouse primary B cells retrovirally expressing mIgG-tail or mIgG-Linker25-tail indicated by the intensity decrease of proliferation marker CFSE. In (c) given is the statistic comparison of the proliferation of primary B cells expressing mIgG-tail or mIgG-Linker25-tail by calculating the mean fluorescence intensity decrease fold of CFSE. Bars indicate mean+s.d. from three independent experiments. Two-tailed t-tests were used for the statistical comparisons. **P<0.01; *P<0.05.