Figure 8: Antigen engagement or Ca2+ mobilization independently facilitates the dissociation of mIgG-tail from the PM.
From: Acidic phospholipids govern the enhanced activation of IgG-B cell receptor
(a–d) Dequenching FRET performed after crosslinking of extracellular domain of mIgG by NP8-BSA in J558L cells expressing B1-8-IgG-BCRs with mIgG-tail (a,b) or mIgG-tail-Y/F (c,d). (e,f) FRET analysis to evaluate the function of Ca2+ influx in dissociating mIgG-tail from the PM in J558L cells expressing B1-8-IgG-BCRs. (g,h) Dequenching FRET analysis of Ramos cells expressing IgG-BCR with mIgG-tail-Y/F in sample buffer with Ca2+ (g) or without Ca2+ (h). For all the analysis, Scale bar, 1.6 μm. Each dot represents one cell analysed in three independent experiments. Bars indicate mean±s.d. Two-tailed t-tests were used for the statistical comparisons. ***P<0.001; **P<0.01; NS, not significant.
