Figure 1: Pathway analysis on the clusters of co-expressed genes.

We ran a co-functionality network analysis on 897 downregulated genes with age (negative effect direction) and 600 upregulated genes with age (positive effect direction) using GeneNetwork. With a correlation threshold of 0.7, we selected all clusters bigger than four genes and ran per-cluster pathway analyses using KEGG, Reactome, and GO-terms in WEBGESTALT. Benjamini & Hochberg FDR was used for multiple testing corrections. The significant threshold 0.05 after correction for multiple testing was applied. (a) Three clusters of downregulated genes with age and (b) four clusters of genes upregulated with age were enriched for functional pathways in KEGG, Reactome, and GO terms; the specific pathways are mentioned next to the (sub)cluster names.