Figure 1: The Snail transgenic skin exhibits increased tissue stiffness.
From: The matrix protein Fibulin-5 is at the interface of tissue stiffness and inflammation in fibrosis
(a) Left: haematoxylin and eosin-stained P9 skin sections. Scale bar, 200 μm. Right: quantitation of dermal thickness. (b) Content of hydroxyproline extracted from the P9 whole skin of WT or Snail Tg mice. (c) WT (left) and Snail transgenic (Snail Tg; right) skin sections from P9 mice were stained for elastin (green) and keratin 5 (K5; red). Scale bar, 100 μm. The right graph shows the quantitation of elastin content in P9 whole skin. (d) Stress–strain curves for P9 WT (blue) and Snail Tg (red) skin. Average tangent modulus of P9 WT and Snail Tg skin at (e) lower strain (0 to 10% strain) and (f) higher strain (20 to 30% strain). All data represent mean±s.d. of at least six samples. *P<0.05, and **P<0.01 as compared with WT. (a–c) Statistical analyses were performed by the unpaired Mann–Whitney U-test. (e,f) Statistical analyses were performed with Student's t-test.
