Figure 2: MAP3K8 controls proliferation, migration and invasion of ovarian cancer cells and tumour growth in mouse xenograft models. | Nature Communications

Figure 2: MAP3K8 controls proliferation, migration and invasion of ovarian cancer cells and tumour growth in mouse xenograft models.

From: MAP3K8/TPL-2/COT is a potential predictive marker for MEK inhibitor treatment in high-grade serous ovarian carcinomas

Figure 2

(a) Left: growth curves of SKOV3 and IGROV-1 ovarian cancer cell lines (OCCLs) treated either with MAP3K8 kinase inhibitor (KI) or with DMSO (vehicle medium for KI), for the indicated times (n=3). Right: bar plots show the doubling times of SKOV3 and IGROV-1 OCCL either untreated (DMSO) or treated with MAP3K8 kinase inhibitor (KI) (n=3). (b) Left: bar plots represent the percentage of SKOV3 and IGROV-1 OCCL in G1, S and G2 phases of the cell cycle, in control condition (DMSO) or following treatment with MAP3K8 KI, for the indicated times. For each cell line, the T0 time point represents the state of the cells just before any treatment (n=3). Right: representative cell-cycle distribution of SKOV3 and IGROV-1 OCCL without (DMSO) or with KI treatment. (c) Bar plots represent cell migration (left panels) or invasion (right panels) of SKOV3 and IGROV-1 OCCL either treated with MAP3K8 KI or with the vehicle medium for KI (DMSO) (n⩾3). (d) Left up: representative western blot showing MAP3K8 protein levels in stable cell lines expressing non-targeting shRNA (shCtrl) or two different MAP3K8-targeting shRNA (shMAP3K8_1 and shMAP3K8_2), derived from SKOV3 and IGROV-1 OCCL, as indicated. Actin is used as an internal control for protein loading. Left down: bar plot showing the ratio of MAP3K8/Actin protein levels, as assessed by densitometry analysis of western blots (as shown above) and expressed as percentage of shCtrl (n=3). Right, Growth curve of stable cell lines (shCtrl, shMAP3K8_1 and shMAP3K8_2) derived from SKOV3 and IGROV-1 OCCL, for the indicated times (n=3). (e) Tumour growth curves over time from one representative experiment of xenografted stable cell lines expressing shCtrl, shMAP3K8_1 or shMAP3K8_2 derived from SKOV3 and IGROV-1 OCCL (N⩾10 tumours per group). For all panels, data are shown as mean±s.e.m. P values are based on a one-sample t-test (c) and on the Student’s t-test (a,b,d,e). *P≤0.05; **P≤0.005 and ***P≤0.0005. n stands for the number of replicated independent experiments.

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