Figure 1: MEX-3C interacts with the major cytoplasmic deadenylation complexes and promotes the shortening of its target mRNA poly(A) tail through its ubiquitin ligase activity. | Nature Communications

Figure 1: MEX-3C interacts with the major cytoplasmic deadenylation complexes and promotes the shortening of its target mRNA poly(A) tail through its ubiquitin ligase activity.

From: A non-proteolytic role for ubiquitin in deadenylation of MHC-I mRNA by the RNA-binding E3-ligase MEX-3C

Figure 1

(a) Gene ontology annotation of proteins binding RINGless MEX-3C. Number of unique proteins is in brackets. Identity of interacting proteins is shown in Supplementary Table 1. (b) MEX-3C interacts with the two mammalian deadenylation complexes CCR4-NOT and PAN2-PAN3. IB, immunoblot. The KH containing RNA-binding protein, PCBP2, was used as negative control. Immunoprecipitations were done in the presence of 20 U ml−1 RNase-I. EV, empty vector/GFP. (c) Shortening of HLA-A2 mRNA’s poly(A) tail is promoted by MEX-3C’s ubiquitin ligase activity. RT–PCR-based Poly(A) tail length assay (PAT) for HLA-A2 mRNA in control (EV/GFP), wild-type or RINGless MEX-3C-expressing (FACS sorted) cells (right panel). ACTIN mRNA was assayed as control (left panel) and Ao PCR controls for loading. Ao: refers to PCR products using primers to amplify the last 100–200 bp of the 3'UTR, excluding the poly(A) tail. NT, no template. (d) Schematic representation of the different components of the mammalian CCR4-NOT and PAN2-PAN3 deadenylation complexes.

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