Figure 2: MEX-3C-mediated degradation of FF-Luc-HLA-A2-3′UTR reporter mRNA is rescued by the depletion of CNOT7/8(Caf1), the major catalytic component of the CCR4-NOT deadenylation complex. | Nature Communications

Figure 2: MEX-3C-mediated degradation of FF-Luc-HLA-A2-3′UTR reporter mRNA is rescued by the depletion of CNOT7/8(Caf1), the major catalytic component of the CCR4-NOT deadenylation complex.

From: A non-proteolytic role for ubiquitin in deadenylation of MHC-I mRNA by the RNA-binding E3-ligase MEX-3C

Figure 2

(a) Depletion of the catalytic components of the deadenylation complexes cannot rescue MEX-3C’s downregulation of HLA-A2 expression. Flow cytometric analysis of cell surface HLA-A2 levels in siRNA-treated HEK293T cells expressing wtMEX-3C or empty vector. GFP is a surrogate marker for MEX-3C expression. For quantification of HLA-A2 protein and mRNA levels, see Supplementary Fig. 2a–b. (b) Depletion of CNOT7/8(Caf1) rescues MEX-3C-mediated degradation of its target mRNA. FF-Luc-HLA-A2-3′UTR mRNA levels were analysed by qRT–PCR. Results are relative to siControl and expressed as mean±s.d. of three independent experiments (n=3). **P-value <0.005 versus siCTRL+MEX-3C; NS, not significant; unpaired Student’s t-test (Supplementary Fig. 2c shows validation of knockdowns).

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