Figure 4: Dab2 excludes classical focal adhesion proteins at RGD-integrin-beta3 clusters. | Nature Communications

Figure 4: Dab2 excludes classical focal adhesion proteins at RGD-integrin-beta3 clusters.

From: Integrin-beta3 clusters recruit clathrin-mediated endocytic machinery in the absence of traction force

Figure 4

(a) Dab2-mCherry and GFP-talin are both recruited to RGD-integrin-beta3 clusters. Dab2 and talin are mutually excluded. (b,c) GFP-Talin is first recruited at RGD clusters, followed by Dab2-mCherry. (c) Time-lapsed images of the boxed area in b. (d) Representative kymograph of mutual exclusion of GFP-talin and Dab2-mCherry at RGD clusters. Dab2 replaces talin and vice versa (arrow). (e) Normalized intensity profiles of Dab2-mCherry and GFP-talin along the white arrow in panel (d). Dab2-mCherry and GFP-talin are inversely correlated. Also see Supplementary Fig. 5. (f–h) Dab2-mCherry is mutually excluded from Kindlin1-GFP, Tensin1-GFP and FilaminA-GFP. Also see Supplementary Movie 4. (i) Pearson’s correlation analysis of Dab2 and adhesion-related adaptor proteins, including Talin, Kindlin1, Tensin1 and FilaminA. Dab2 and adhesion-related adhesion proteins are inversely correlated. All data are the mean of three independent experiments (n>20 in each condition). Error bars represent s.d., and all samples represent biological replicates. Detailed statistical results are available in Supplementary Table 1. Scale bar, 5 μm.

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