Figure 1: Flow chart indicating study design.
From: A CpG-methylation-based assay to predict survival in clear cell renal cell carcinoma
We identified candidate CpGs sites from 46 paired ccRCC and adjacent normal tissues by CpG methylation microarray in the discovery set. We then used a multivariate LASSO Cox regression model to build a CpG-based prognostic classifier in SYSU set. Furthermore, the five-CpG-based classifier was validated in MCHC, UTSW and TCGA data sets. Relationship between CpG methylation, gene expression and patient prognosis were also analysed in the TCGA set.
