Figure 2: POH1 depletion in primary liver cells decreases E2F1 protein levels.
From: POH1 deubiquitylates and stabilizes E2F1 to promote tumour formation
(a) Schematic diagram of the wild-type and the floxed poh1 alleles. Mice with a floxed poh1 allele were crossed with a Cre line to generate deleted poh1 allele. (b) PCR analysis of genomic DNA demonstrating that the floxed poh1 alleles are efficiently deleted in Mx-Cre+ and poh1f/f mouse liver tissues. (c) POH1 and E2F1 immunoblotting in liver tissues from poh1w/w, Mx-Cre+ and poh1f/f, Mx-Cre+ mice treated with three rounds of polyI:C injections. (d) IHC analysis of POH1 and E2F1 expression in liver tissues. Representative images are presented. Scale Bar, 100 μm. (e) Cell lysates of mouse liver tissues with (poh1f/f, Mx-Cre+) or without (poh1w/w, Mx-Cre+) POH1 deletion were immunoprecipitated with anti-E2F1 antibody, and the immunocomplexes were immunoblotted with indicated antibodies.
