Figure 2: Impact of gatastatin on MT regrowth in HeLa cells. | Nature Communications

Figure 2: Impact of gatastatin on MT regrowth in HeLa cells.

From: The γ-tubulin-specific inhibitor gatastatin reveals temporal requirements of microtubule nucleation during the cell cycle

Figure 2

(a) The impact of gatastatin on MT nucleation by centrosomes in interphase. After nocodazole and ice treatments, MT nucleation (1 min at 37 °C) in the presence of 30 μM of gatastatin or the DMSO solvent control was monitored. Red, green and blue in the merged image represent EB1, α-tubulin and pericentrin, respectively. Scale bar, 10 μm. Three independent experiments were performed and one representative experiment is shown. (b) The area of EB1 around centrosomes in a was calculated using the ImageJ software from 97 (control) or 84 (gatastatin) centrosomes. Error bars represent s.e.m. Two-tailed, unpaired Student’s t-test was used to obtain P value. (c) The impact of gatastatin on MT nucleation by centrosomes in mitosis. After STLC and ice treatments, spindle MT nucleation (1 min at 20 °C) in the presence of 30 μM of gatastatin or the DMSO solvent control was monitored. Red, green and blue in merged image represent EB1, α-tubulin and pericentrin, respectively. Scale bar, 10 μm. Three independent experiments were performed and one representative experiment is shown. (d) The area of EB1 around centrosomes in c was calculated using ImageJ software from 22 (control) or 17 (gatastatin) monopolar spindles. Error bars represent s.e.m. Two-tailed, unpaired Student’s t-test was used to obtain P value.

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