Figure 5: Impact of gatastatin on anaphase spindle elongation. | Nature Communications

Figure 5: Impact of gatastatin on anaphase spindle elongation.

From: The γ-tubulin-specific inhibitor gatastatin reveals temporal requirements of microtubule nucleation during the cell cycle

Figure 5

γ-Tubulin function is important for the anaphase progression. (a) Scheme of the experiment in b–d. After washout of MG132 from 30 μM gatastatin-treated metaphase cells, the transition into anaphase, telophase or cytokinesis was observed in the presence of reversine. (b) Anaphase duration in a was analysed from 14 cells with a × 40 objective. Error bars represent s.e.m. Two-tailed, unpaired Student’s t-test was used to obtain P value. Three independent experiments were performed and one representative data set is shown. (c) Anaphase spindle and midzone structure of HeLa cells expressing mCherry-H2B and EGFP-α-tubulin in the presence of 1% DMSO or 30 μM gatastatin was observed with a × 60 objective. Green and orange represent α-tubulin and DNA, respectively. Arrowheads and arrows represent anaphase spindle and midzone, respectively. Scale bar, 5 μm. (d) The anaphase spindle and midzone structures in b were categorized into two patterns as shown in c.

Back to article page