Figure 3: Mod(mdg4)-like trans-splicing events conserved in insects.

(a) Two different types of mod(mdg4)-like trans-splicing. AA, number of amino acids; Red bar, donor transcript (5′-common exons); blue bar, acceptor transcript (3′-alternative exons). Arrows denote direction of transcripts. *Assuming that scaffold_278 and scaffold_162 are located on the same chromosome in P. xylostella. (b) Diagram of the mod(mdg4) locus in D. plexippus. Red bar, donor transcript (5′-common exons); blue bar, acceptor transcript (3′-alternative exons). Black arrows indicate strand direction. Mod(mdg4) isoforms validated by RT–PCR are marked by orange arrows. The RT–PCR is performed as described in Methods using mixed D. plexippus samples (n=3). The name of each acceptor is denoted by DPOGS20 (prefix) plus the four-digit label of blue bar(s).