Figure 4: Sequence diversification of mod(mdg4) products throughout evolution.

(a) Exon structure of the mod(mdg4) N-terminus. The BTB domain-encoding region is marked by a dashed box. Grey box, exon. (b) Sequence similarity of N-terminal regions. Number of conserved amino acid at each position is indicated by blue curve. Sequences are aligned based on the BTB domain model using HMMalign⁵⁹ (see Methods for details). Only alignment of the conserved region is shown. Dme, D. melanogaster; Aae, A. aegypti; Aga, A. gambiae; Bmo, B. mori; Dpl, D. plexippus; Pxy, P. xylostella; Dpu, D. pulex. (c) Phylogenetic analysis of C-terminal FLYWCH domains using Maximum Likelihood analysis (see Methods for details). FLYWCH domains from different species are marked in distinct colours. The Dipteran and Lepidopteran groups are separated by different background colours. The same tree with detailed labelling is provided in Supplementary Fig. 5.