Figure 3: Caspase-4 and -5 are required for IL-1α and IL-1β release. | Nature Communications

Figure 3: Caspase-4 and -5 are required for IL-1α and IL-1β release.

From: Human caspase-4 and caspase-5 regulate the one-step non-canonical inflammasome activation in monocytes

Figure 3

(a) Monocytes were treated with either Z-YVAD-fmk (caspase-1 inhibitor) or LEVD-fmk (caspase-4/5 inhibitor) for 1 h before LPS stimulation. Culture supernatants were collected after 5 h and secretion of IL-1α, IL-1β and IL-6 was determined by ELISA. (b,e) Silencing of caspase-4 (b) or caspase-5 (d) was assessed by western blot in siRNA-treated monocytes stimulated with LPS. GAPDH was used as loading control. Densitometry analysis of pro-caspase-5 and caspase-5 p20 was also shown. (c,e) Cytokine release from siRNA-treated monocytes following LPS stimulation was evaluated by ELISA. Blots are representative of three independent experiments. Graphs show the mean±s.d. of triplicate wells, and are representative of three or more independent experiments. *P value<0.05, **P value<0.01.

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