Figure 5: Syk kinase and Syk-mediated endocytosis of LPS trigger caspase-5 processing and cytokine release. | Nature Communications

Figure 5: Syk kinase and Syk-mediated endocytosis of LPS trigger caspase-5 processing and cytokine release.

From: Human caspase-4 and caspase-5 regulate the one-step non-canonical inflammasome activation in monocytes

Figure 5

(a,b) Western blot analysis of p-Syk (Tyr 525–526) or total Syk in monocytes that were either left untreated (UT) or stimulated with LPS, LPS-RE or lipid A for the indicated time-points. (c,d) Western blot analysis of caspase-5 and caspase-5 p20 in monocytes pre-treated for 1 h with the Syk inhibitor R406 (0.5–2 μM) (c) or the dynamin inhibitor dynasore (40–80 μM) (d) prior stimulation with LPS for 5 h. Blots are representative of three independent experiments. Densitometry analyses of caspase5 p20 are also shown (right). (e,f) Release of IL-1α, IL-1β and IL-6 from monocytes pre-treated with R406 (e) or dynasore (f) before LPS stimulation. Graphs (e,f) show the mean±s.d. of triplicate wells and are representative of three independent experiments. Graphs (c,d) (right) show the mean±s.d. of three independent experiments and significance evaluated using a one sample t-test. *P value<0.05, **P value<0.01.

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