Figure 3: Cleavage of APP by AEP at N585 accelerates Aβ production. | Nature Communications

Figure 3: Cleavage of APP by AEP at N585 accelerates Aβ production.

From: Delta-secretase cleaves amyloid precursor protein and regulates the pathogenesis in Alzheimer’s disease

Figure 3

(a) Schematic drawing of APP cleavage by AEP, β-secretase and α-secretase. (b,c) Western blot (WB) showing the processing of APP fragments by α- and β-secretase. Arrows indicate APP C-terminal fragments generated by BACE1 (C99, b) and ADAM10 (C83, c). The percentage of C99 and C83 was quantified. (d–g) ELISA results showing the concentrations of Aβ 1–40 and Aβ 1–42 in medium from AEP+/+ and AEP−/− primary neurons (d), HEK293 cells stably transfected with APP (e), HEK293 cells transiently transfected with GST-tagged APP fragments (f) or GFP-tagged mutant APP (g). The binding of APP fragments with BACE1 was analysed using GST pull-down assay (f). The internalized APP was assessed by internalization assay (g). Data are mean±s.e.m. of three independent experiments, and were analysed using one-way analysis of variance followed by post hoc comparison, *P<0.01.

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