Figure 5: The AEP-fragmented APP1–373 induces neurodegeneration in primary cultured neurons. | Nature Communications

Figure 5: The AEP-fragmented APP1–373 induces neurodegeneration in primary cultured neurons.

From: Delta-secretase cleaves amyloid precursor protein and regulates the pathogenesis in Alzheimer’s disease

Figure 5

(a) Western blotting (WB) of the purified recombinant APP fragments. (b) Representative image of MAP2 and TUNEL staining after the neurons were incubated with APP fragments. Scale bar, 20 μm. (c) Percentage of neurons with abnormal morphology. Percentage of TUNEL-positive neurons induced by incubating with 10 μg ml−1 recombinant APP fragments (d) or different dose of APP1–373 fragment (e). Percentage of TUNEL-positive cells after PC12 cells (f) or HEK293 cells (g) were incubated with 10 μg ml−1 APP1–373 fragment (mean±s.e.m.). (h) Percentage of TUNEL-positive neurons after infected with lentivirus encoding APP fragments. Data are mean±s.e.m. of three independent experiments, and were analysed using t-test (f,g) or one-way analysis of variance followed by post hoc comparison (c,d,e,h), *P<0.01.

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