Figure 6: Biological relevance of Nek7 allosteric activation by Nek9.
From: Mechanistic basis of Nek7 activation through Nek9 binding and induced dimerization
(a,b) HeLa cells were transfected with RNAi resistant Flag-Nek7 WT or binding-deficient mutants as indicated, and grown in selection media containing G418 for 7 days to enrich the transfected cell population. Cells were then either mock- or Nek7-depleted for 72 h before being fixed and processed for immunofluorescence microscopy with Flag and phospho-H3 antibodies. The mitotic index of transfected cells was counted. Data represent mean (±s.d.) for three separate experiments where n=100–200 cells. Statistical analyses were carried out using a one-way analysis of variance followed by post hoc testing. *P<0.05, **P<0.01 and ***P<0.005.
