Figure 2: Time-lapse microscopy in the presence of F10 concentrations.
From: Microscale insights into pneumococcal antibiotic mutant selection windows
(a) Still images (phase contrast microscopy) after 1, 3, 5, 10 and 20 h of a time-lapse experiment of S. pneumoniae D-PEP22 cells growing out to form microcolonies (and subsequent autolysis) on a semisolid surface without antibiotics. (b) Tracking of D-PEP22 cells treated with bacteriostatic antibiotics at the F10 level. Note the large amount of cell-to-cell variability, such as in case of rifampicin, where two cells develop in opposing directions (marked with arrows), with the progeny of one lysing while the other one grows out successfully. (c) Tracking of D-PEP22 cells treated with bactericidal antibiotics at the F10 level. CHL, chloramphenicol; CIP, ciprofloxacin; ERY, erythromycin; KAN, kanamycin; RIF, rifampicin; STR, streptomycin; TET, tetracycline; TMP, trimethoprim. Scale bar, 5 μm.
