Figure 4: Immunodetection of pendrin and/or β-galactosidase.
From: KCNK5 channels mostly expressed in cochlear outer sulcus cells are indispensable for hearing
In cryostat sections from adult cochleas, pendrin (yellow) appears in spiral prominence (Sp) and root cells (Rc) with a similar pattern of expression in Kcnk5+/− (a) and Kcnk5−/− (b) mice at P45. DAPI (4′,6-diamidino-2-phenylindole dihydrochoride) staining of cell nuclei (blue). In adult Kcnk5+/−mouse cochlea (c–k), β-galactosidase (green) mainly found in Claudius’ cells (Cc, c,d,i,j), is also present in Rc (e,k), while pendrin (magenta) is localized in Rc (c–h). Merged images (c–e) show that pendrin and β-galactosidase only colocalize in Rc. Stacks of 23 confocal planes of 1.8 μm each in c, f and i, single confocal planes of 1.8 μm optic thickness in d, g and j and single confocal planes of 0.7 μm optic thickness in e, h and k. Scale bars, 50 μm (a–c,f,i); 20 μm (d,e,g,h,j,k).
