Figure 5: Immunodetection of KCNJ10 and KCNQ1 in adult Kcnk5+/− and Kcnk5−/− mouse cochleas.
From: KCNK5 channels mostly expressed in cochlear outer sulcus cells are indispensable for hearing
(a–d) In both mice groups, KCNJ10 (green) is expressed in the intermediate cells (a,b), while KCNQ1 (green) is located in the marginal cells (c,d) of the stria vascularis. Confocal images (stacks of 5 and 2 confocal planes of 2 μm each, respectively, in e and f) in the area of the root cells (e–j) revealed similar colocalizations of KCNJ10 (green) and tubulin (magenta) in Kcnk5+/− (e,g,h) and Kcnk5−/− (f,i,j) mice. Cells’ nuclei are stained with 4′,6-diamidino-2-phenylindole dihydrochoride (DAPI) (blue, a–j). Scale bars, 50 μm (a–d); 20 μm (e–j).
