Figure 4: GLB-12 shows a distinct tissue and subcellular expression pattern.
From: A redox signalling globin is essential for reproduction in Caenorhabditis elegans
(a) GLB-12 is membrane-bound, as seen in head neurons. Upper scale bar: 100 μm; lower scale bar, 10 μm. The graphic shows the presence of a myristoylation and palmitoylation site in the N-terminal region (N: N-terminal, C: C-terminal, AA: amino acid). (b) Deletion of the myristoylation or palmitoylation site disrupts cell membrane localization, as seen in human neuroblastoma SH-SY5Y cells. Scale bar: 10 μm. (c) A cytosolic RNAi-resistant (RR) GLB-12 is not capable of rescuing the glb-12 RNAi reduction in fecundity (n=5). Percentages show control RNAi compared to glb-12 RNAi within a strain, *P<0.05; **P<0.01. Data are mean±s.e.m.
