Figure 2: RbTR and p53TR alleles phenocopy conventional floxed and knockout alleles.
From: Recombinase-based conditional and reversible gene regulation via XTR alleles
(a) Number of Rb+/+, RbTR/+ and RbTR/TR newborn pubs observed in RbTR/+X RbTR/+ crosses. Percentage and number indicated, χ2=12.4, df=2, P=10−4. (b) GFP detection in RbTR/TR, RbTR/+ and Rb+/+ embryonic day 13.5 embryos. (c) Kaplan–Meier analysis of lymphoma onset in tamoxifen-treated Eμ-Myc; p53XTR/XTR; Rosa26CreER/+ and Eμ-Myc; p53+/+; Rosa26CreER/+mice; P=0.0017, log-rank (Mantel–Cox) test. (d) GFP imaging of lymphoma cells in an Eμ-Myc; p53XTR/XTR; Rosa26CreER/+ mouse after lymphoma onset. (e) Initiation of sarcomas by intramuscular (IM) injection of AdCre into the hindlimb of KrasLSL-G12D/+; p53XTR/XTR(KPXTR/XTR) and KrasLSL-G12D/+; p53flox/flox(KPflox/flox) mice. (f) Kaplan–Meier analysis of sarcoma onset in KPXTR/XTR and KPflox/flox mice. (g) Representative sarcomas from KPXTR/XTR (n=11) and KPflox/flox (n=3) mice shown by whole-mount bright-field and fluorescent (GFP) microscopy and also (haematoxylin and eosin) staining of histological sections. (h) Initiation of lung adenocarcinoma by inhalation of AdCre in KPXTR/XTR (n=8) and KPflox/flox (n=10) mice. (i) Kaplan–Meier survival analysis in KPXTR/XTR and KPflox/flox mice after inhalation of AdCre. (j) Representative lungs from KPXTR/XTR and KPflox/flox mice shown by whole-mount bright-field and fluorescent (GFP) microscopy and H&E staining of histological sections. (k,l,m) Comparison of tumour burden (% of lung area), tumour number and tumour grade between KPXTR/XTR and KPflox/floxmice. Scale bars, 25 μm.
