Table 1 List of antibodies used for immunostaining and/or FACS analysis.

From: Propagation of oestrogen receptor-positive and oestrogen-responsive normal human breast cells in culture

Antibody

Clone

Company/Catalogue No.

Peroxidase

Fluorescence

FACS

AP2β

Santa Cruz, sc-8976

1:50

BCL2

124

Dako, M0887

1:25

CDw75

LN1

NeoMarkers, MS-130-P

1:10

CD117

104D2

Dako, M7140

1:50

CD117

K44.2

Sigma, K0131

1:50

CD117, PE

104D2

BD Biosciences, 332785

1:20

CD166

3A6

BioLegend, 343902

1:50

CD166, Alexa Fluor 488

3A6

AbD Serotec, MCA1926A488

1:20

CD271, APC

ME20.4

Cedarlane, CL10013APC

1:50

CD326, PerCP/Cy5.5

9C4

BioLegend, 324214

1:10–1:20

TGFβRII

MM0056–4F14

Abcam, ab78419

1:20

ERα

SP1

Labvision, RM-9101-S

1:25

1:10

ERα

SP1

Labvision, RM-9101-R7

Prediluted, ready to use

ERα

1D5

Dako, M7047

1:100

1:25

GATA3

HG3–31

Santa Cruz, sc-268

1:25

Keratin 8

M20

Abcam, ab9023

1:50

1:50

Keratin 8

TS1

Novocastra, NCL-CK8-TS1

1:50

Keratin 20*

Ks20.8

Dako, M7019

1:25

1:10

Keratin 20*

Ks20.8

Dako, IR777

Prediluted, ready to use

Keratin 20*

Ks20.8

Abcam, ab854

1:10

Keratin 20*

Ks20.8

Genemed, 61–0018–2

1:25

Keratin 20*

Ks20.8

Santa Cruz, SC-52320

1:25

Keratin 20

IT-Ks20.10

Progen Biotechnik, 61054

1:25

Keratin 20

CK205

Novocastra, NCL-CK20–543

1:50

Keratin 8/18

NCL-5D3

Abcam, ab90102

1:50

Keratin 14

LL002

NeoMarkers, MS-115-P

1:300

1:25

Keratin 15

LHK15

NeoMarkers, MS-1068-P

1:25

Keratin 18

M9

Monosan, MON3006

1:100

N-cadherin

32

BD Transduction Laboratories, 610920

1:25

PR

SAN27

Vector Laboratories, VP-P987

1:100

1:25

PR

SP2

Labvision, RM-9102-S

1:10

PR

PgR 636

Dako, M356901–2

1:50

1:10

 

67-kDa laminin receptor

MLuC5

Abcam, ab3099

1:50

1:50

  1. *Originally described to recognize cytokeratin 20, but keratin 20 is not expressed in normal human breast14.